Journal: Nutrients

Article Title: Agarwood Oil Nanoemulsion Attenuates Cigarette Smoke-Induced Inflammation and Oxidative Stress Markers in BCi-NS1.1 Airway Epithelial Cells

doi: 10.3390/nu15041019

Figure Lengend Snippet: Effect of Agarwood-NE on the CSE-induced transcription of the pro-inflammatory cytokine IL-8. BCi-NS1.1 cells were pre-incubated for 1 h in the presence of 25 and 50 µg/mL agarwood-NE, followed by exposure to 5% CSE for 24 h. The mRNA levels of IL-8 were determined via RT-qPCR. Values are expressed as mean ± SEM ( n = 4, *: p < 0.05; ****: p < 0.0001).

Article Snippet: The effects of agarwood-NE on cytokine expression levels in CSE-induced BCiNS1.1 cells were assessed using a human cytokine protein array kit (R&D Systems, Minneapolis, MN, USA), as described in a previous study [ ].

Techniques: Incubation, Quantitative RT-PCR

Journal: Nutrients

Article Title: Agarwood Oil Nanoemulsion Attenuates Cigarette Smoke-Induced Inflammation and Oxidative Stress Markers in BCi-NS1.1 Airway Epithelial Cells

doi: 10.3390/nu15041019

Figure Lengend Snippet: Effect of Agarwood-NE on the CSE-induced production of pro-inflammatory mediators in human cytokine protein array. BCi-NS1.1 cells were pre-incubated for 1 h in the presence of 25 and 50 µg/mL agarwood-NE, followed by exposure to 5% CSE for 24 h. The protein levels of IL-1α ( A ), IL-1β ( B ), IL-1Ra ( C ), and GDF-15 ( D ) were determined via human cytokine protein array.

Article Snippet: The effects of agarwood-NE on cytokine expression levels in CSE-induced BCiNS1.1 cells were assessed using a human cytokine protein array kit (R&D Systems, Minneapolis, MN, USA), as described in a previous study [ ].

Techniques: Protein Array, Incubation

Journal: Nutrients

Article Title: Agarwood Oil Nanoemulsion Attenuates Cigarette Smoke-Induced Inflammation and Oxidative Stress Markers in BCi-NS1.1 Airway Epithelial Cells

doi: 10.3390/nu15041019

Figure Lengend Snippet: Effect of agarwood-NE on the CSE-inhibited production of anti-inflammatory mediators in human cytokine protein array. BCi-NS1.1 cells were pre-incubated for 1 h in the presence of 25 and 50 µg/mL agarwood-NE, followed by exposure to 5% CSE for 24 h. The protein levels of IL-10 ( A ), IL-18Bpa ( B ), GH ( C ), VDBP ( D ), relaxin-2 ( E ), IFN-γ ( F ), PDGF-BB ( G ), and TFF3 ( H ) were determined via human cytokine protein array.

Article Snippet: The effects of agarwood-NE on cytokine expression levels in CSE-induced BCiNS1.1 cells were assessed using a human cytokine protein array kit (R&D Systems, Minneapolis, MN, USA), as described in a previous study [ ].

Techniques: Protein Array, Incubation

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Lipoxin Signaling in Murine Lung Host Responses to Cryptococcus neoformans Infection

doi: 10.1165/rcmb.2014-0102OC

Figure Lengend Snippet: Strain-specific cytokine production in response to lung Cne infection. Cytokine profiles of naive and Cne-infected (Day 14 after infection) C.B-17 (open bars) and C57BL/6 (solid bars) mice were measured in BALF by cytokine bead array (see Materials and Methods). Cytokines analyzed included IL-17 (A), IFN-γ (B), IL-4 (C), IL-13 (D), TNF-α (E), and IL-1β (F). Data are presented as mean ± SEM (n = 5 per group). *P ≤ 0.01 for comparisons between Day 0 versus Day 14 within a strain. #P ≤ 0.01 for comparisons between strains at a given time point. Data were analyzed by Student’s t tests for differences within and between strains at specific time points.

Article Snippet: Aliquots of bronchoalveolar lavage fluid (BALF) were submitted to Aushon for multiplex cytokine immunoassay (SearchLight Chemiluminescent Protein Array; Aushon, Billerica, MA).

Techniques: Infection